细胞趋硬性迁移的研究进展

杨月华; 姜洪源

doi:10.21656/1000-0887.420265

细胞趋硬性迁移的研究进展

doi: 10.21656/1000-0887.420265

杨月华,
姜洪源^,

中国科学技术大学工程科学学院近代力学系中国科学院材料力学行为和设计重点实验室，合肥 230026

基金项目:

国家自然科学基金（12002338；12025207；11872357）

中央高校基本科研业务费(WK2090000017)

安徽省自然科学基金（2008085QA24）

统筹推进世界一流大学和一流学科建设专项资金(YD2480002001)

详细信息

作者简介:
杨月华（1992—），男，特任副研究员(E-mail: hz293033@ustc.edu.cn);姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

通讯作者:
姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

中图分类号: O3
计量
- 文章访问数: 649
- HTML全文浏览量: 90
- PDF下载量: 126
- 被引次数: 0
出版历程
- 收稿日期: 2021-09-03
- 修回日期: 2021-09-10

Research Advances in Cell Durotaxis

YANG Yuehua,
JIANG Hongyuan^,

CAS Key Laboratory of Mechanical Behavior and Design of Materials，Department of Modern Mechanics, School of Engineering Science, University of Science and Technology of China, Hefei 230026, P.R.China

Funds:

The National Natural Science Foundation of China(12002338

12025207

11872357)

摘要

摘要: 细胞力学微环境可以调控许多细胞生理功能.特别地，在细胞力学微环境各种信号梯度的作用下，细胞可以定向地迁移.这些定向迁移可以显著影响伤口愈合、癌细胞转移和组织形貌发育等生理过程.目前为止，细胞的定向迁移主要包括：在化学药物梯度作用下的趋药性迁移，在黏附分子梯度作用下的趋触性迁移，以及在细胞外基质硬度梯度作用下的趋硬性迁移等.虽然细胞趋药性和趋触性迁移的力学机理得到了很好的研究，但是关于细胞趋硬性迁移的机制和作用还不清楚.该文重点介绍了细胞趋硬性的相关实验和理论研究进展，分析了不同研究间的联系与区别，讨论了细胞趋硬性迁移的潜在力学机制，提出尚存在的问题和未来可能的研究方向.
- 细胞力学微环境 /
- 细胞定向迁移 /
- 趋硬性迁移 /
- 体积变化 /
- 细胞极性
Abstract: The cellular mechanical microenvironment can regulate many cellular physiological functions. In particular, cells can migrate directionally under external cue gradients from their mechanical microenvironment. These directed migrations play critical roles in wound healing, cancer cell metastasis, and tissue morphology development. So far, directional cell migration mostly includes: the directional migration under chemical gradients (chemotaxis), the directional migration under adhesion gradients (haptotaxis), and the directional migration under mechanical gradients (durotaxis). Although the basic mechanisms of chemotaxis and haptotaxis are well characterized, the mechanism of durotaxis remains unclear. In this review, we describe the experimental and theoretical advances in the study of cell durotaxis, analyze the connections and differences among different studies, discuss the potential mechanical mechanism of cell durotaxis, and put forward the remaining problems and possible future research directions.
- cellular mechanical microenvironment /
- directional cell migration /
- cell durotaxis /
- volume regulation /
- cell polarity

HTML全文

参考文献(63)

FRIEDL P, GILMOUR D. Collective cell migration in morphogenesis, regeneration and cancer[J].Nature Reviews Molecular Cell Biology,2009,10(7): 445-457.

[2]ROCA-CUSACHS P, SUNYER R, TREPAT X. Mechanical guidance of cell migration: lessons from chemotaxis[J].Current Opinion in Cell Biology,2013,25(5): 543-549.

[3]KOSER D E, THOMPSON A J, FOSTER S K, et al. Mechanosensing is critical for axon growth in the developing brain[J].Nature Neuroscience,2016,19(12): 1592-1598.

[4]TOZLUOGLU M, TOURNIER A L, JENKINS R P, et al. Matrix geometry determines optimal cancer cell migration strategy and modulates response to interventions[J].Nature Cell Biology,2013,15(7): 751-762.

[5]DONA E, BARRY J D, VALENTIN G, et al. Directional tissue migration through a self-generated chemokine gradient[J].Nature,2013,503: 285-289.

[6]GUNAWAN R C, SILVESTRE J, GASKINS H R, et al. Cell migration and polarity on microfabricated gradients of extracellular matrix proteins[J].Langmuir,2006,22(9): 4250-4258.

[7]WEBER M, HAUSCHILD R, SCHWARZ J, et al. Interstitial dendritic cell guidance by haptotactic chemokine gradients[J].Science,2013,339(6117): 328-332.

[8]SMITH J T, TOMFOHR J K, WELLS M C, et al. Measurement of cell migration on surface-bound fibronectin gradients[J].Langmuir,2004,20(19): 8279-8286.

[9]ZHU M, TAO H, SAMANI M, et al. Spatial mapping of tissue properties in vivo reveals a 3D stiffness gradient in the mouse limb bud[J].Proceedings of the National Academy of Sciences,2020,117(9): 4781-4791.

[10]LO C M, WANG H B, DEMBO M, et al. Cell movement is guided by the rigidity of the substrate[J].Biophysical Journal,2000,79(1): 144-152.

[11]DUCHEZ B J, DOYLE A D, DIMITRIADIS E K, et al. Durotaxis by human cancer cells[J].Biophysical Journal,2019,116(4): 670-683.

[12]VINCENT L G, CHOI Y S, ALONSO-LATORRE B, et al. Mesenchymal stem cell durotaxis depends on substrate stiffness gradient strength[J].Biotechnology Journal,2013,8(4): 472-484.

[13]HARTMAN C D, ISENBERG B C, CHUA S G, et al. Vascular smooth muscle cell durotaxis depends on extracellular matrix composition[J].Proceedings of the National Academy of Sciences,2016,113(40): 11190-11195.

[14]ZAARI N, RAJAGOPALAN P, KIM S K, et al. Photopolymerization in microfluidic gradient generators: microscale control of substrate compliance to manipulate cell response[J].Advanced Materials,2004,16(23/24): 2133-2137.

[15]YANG Y, XIE K, JIANG H. Durotaxis index of 3T3 fibroblast cells scales with stiff-to-soft membrane tension polarity[J].Biophysical Journal,2020,119(7): 1427-1438.

[16]WONG J Y, VELASCO A, RAJAGOPALAN P, et al. Directed movement of vascular smooth muscle cells on gradient-compliant hydrogels[J].Langmuir,2003,19(5): 1908-1913.

[17]ISENBERG B C, DIMILLA P A, WALKER M, et al. Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength[J].Biophysical Journal,2009,97(5): 1313-1322.

[18]CHOI Y S, VINCENT L G, LEE A R, et al. The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices[J].Biomaterials,2012,33(29): 6943-6951.

[19]HADDEN W J, YOUNG J L, HOLLE A W, et al. Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels[J].Proceedings of the National Academy of Sciences,2017,114(22): 5647-5652.

[20]MCKENZIE A J, HICKS S R, SVEC K V, et al. The mechanical microenvironment regulates ovarian cancer cell morphology, migration, and spheroid disaggregation[J].Scientific Reports,2018,8(1): 7228.

[21]LACHOWSKI D, CORTES E, PINK D, et al. Substrate rigidity controls activation and durotaxis in pancreatic stellate cells[J].Scientific Reports,2017,7(1): 2506.

[22]LEVENTAL K R, YU H, KASS L, et al. Matrix crosslinking forces tumor progression by enhancing integrin signaling[J].Cell,2009,139(5): 891-906.

[23]ISOMURSU A, PARK K-Y, HOU J, et al. Negative durotaxis: cell movement toward softer environments[R/OL]. 2020. DOI: 10.1101/2020.10.27.357178.

[24]SUNYER R, CONTE V, ESCRIBANO J, et al. Collective cell durotaxis emerges from long-range intercellular force transmission[J].Science,2016,353(6304): 1157-1161.

[25]MARTINEZ J S, SCHLENOFF J B, KELLER T C S. Collective epithelial cell sheet adhesion and migration on polyelectrolyte multilayers with uniform and gradients of compliance[J].Experimental Cell Research,2016,346(1): 17-29.

[26]SUNYER R, TREPAT X. Durotaxis[J].Current Biology,2020,30(9): R383-R387.

[27]TEE S-Y, FU J, CHEN C S, et al. Cell shape and substrate rigidity both regulate cell stiffness[J].Biophysical Journal,2011,100(5): L25-L27.

[28]LEIPZIG N D, SHOICHET M S. The effect of substrate stiffness on adult neural stem cell behavior[J].Biomaterials,2009,30(36): 6867-6878.

[29]LIU N, ZHOU M, ZHANG Q, et al. Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells[J].Cell Proliferation,2018,51(5): 12478.

[30]ENGLER A J, SEN S, SWEENEY H L, et al. Matrix elasticity directs stem cell lineage specification[J].Cell,2006,126(4): 677-689.

[31]CHAUDHURI O, GU L, KLUMPERS D, et al. Hydrogels with tunable stress relaxation regulate stem cell fate and activity[J].Nature Materials,2016,15(3): 326-334.

[32]XIE K, YANG Y, JIANG H. Controlling cellular volume via mechanical and physical properties of substrate[J].Biophysical Journal,2018,114(3): 675-687.

[33]GALBRAITH C G, YAMADA K M, GALBRAITH J A. Polymerizing actin fibers position integrins primed to probe for adhesion sites[J].Science,2007,315(5814): 992-995.

[34]KAWANO T, KIDOAKI S. Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels[J].Biomaterials,2011,32(11): 2725-2733.

[35]WONG S, GUO W H, WANG Y L. Fibroblasts probe substrate rigidity with filopodia extensions before occupying an area[J].Proceedings of the National Academy of Sciences,2014,111(48): 17176-17181.

[36]CHAO P G, SHENG S C, CHANG W R. Micro-composite substrates for the study of cell-matrix mechanical interactions[J].Journal of the Mechanical Behavior of Biomedical Materials,2014,38: 232-241.

[37]RAAB M, SWIFT J, DINGAL P C D P, et al. Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-Ⅱ heavy chain[J].The Journal of Cell Biology,2012,199(4): 669-683.

[38]BRECKENRIDGE M T, DESAI R A, YANG M T, et al. Substrates with engineered step changes in rigidity induce traction force polarity and durotaxis[J].Cellular and Molecular Bioengineering,2014,7: 26-34.

[39]HAN S J. Spatial and temporal coordination of traction forces in one-dimensional cell migration[J].Cell Adhesion & Migration,2016,10(5): 529-539.

[40]WANG H B, DEMBO M, HANKS S K, et al. Focal adhesion kinase is involved in mechanosensing during fibroblast migration[J].Proceedings of the National Academy of Sciences,2001,98(20): 11295-11300.

[41]ZMURCHOK C, COLLETE J, RAJAGOPAL V, et al. Membrane tension can enhance adaptation to maintain polarity of migrating cells[J].Biophysical Journal,2020,119(8): 1617-1629.

[42]WINKLER B, ARANSON I S, ZIEBERT F. Membrane tension feedback on shape and motility of eukaryotic cells[J].Physica D: Nonlinear Phenomena,2016,318/319: 26-33.

[43]HOUK A R, JILKINE A, MEJEAN C O, et al. Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration[J].Cell,2012,148(1/2): 175-188.

[44]LIEBER A D, SCHWEITZER Y, KOZLOV M M, et al. Front-to-rear membrane tension gradient in rapidly moving cells[J].Biophysical Journal,2015,108(7): 1599-1603.

[45]HETMANSKI J H R, DE BELLY H, BUSNELLI I, et al. Membrane tension orchestrates rear retraction in matrix-directed cell migration[J].Developmental Cell,2019,51(4): 460-475.

[46]BATCHELDER E L, HOLLOPETER G, CAMPILLO C, et al. Membrane tension regulates motility by controlling lamellipodium organization[J].Proceedings of the National Academy of Sciences,2011,108(28): 11429-11434.

[47]WEI C, WANG X, ZHENG M, et al. Calcium gradients underlying cell migration[J].Current Opinion in Cell Biology,2012,24(2): 254-261.

[48]MISSIRLIS D, SPATZ J P. Combined effects of PEG hydrogel elasticity and cell-adhesive coating on fibroblast adhesion and persistent migration[J].Biomacromolecules,2014,15(1): 195-205.

[49]MAIURI P, RUPPRECHT J-F, WIESER S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence[J].Cell,2015,161(2): 374-386.

[50]HOUSE D, WALKER M L, WU Z, et al. Tracking of cell populations to understand their spatio-temporal behavior in response to physical stimuli[J].IEEE,2009,978(1): 187-193.

[51]YU G, FENG J, MAN H, et al. Phenomenological modeling of durotaxis[J].Physical Review E,2017,96(1): 010402.

[52]DOERING C R, MAO X, SANDER L M. Random walker models for durotaxis[J].Physical Biology,2018,15(6): 066009.

[53]NOVIKOVA E A, RAAB M, DISCHER D E, et al. Persistence-driven durotaxis: generic, directed motility in rigidity gradients[J].Physical Review Letters,2017,118(7): 078103.

[54]PEYTON S R, PUTNAM A J. Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion[J].Journal of Cellular Physiology,2005,204(1): 198-209.

[55]BERSHADSKY A D, BALABAN N Q, GEIGER B. Adhesion-dependent cell mechanosensitivity[J].Annual Review of Cell and Developmental Biology,2003,19(1): 677-695.

[56]SHEMESH T, GEIGER B, BERSHADSKY A D, et al. Focal adhesions as mechanosensors: a physical mechanism[J].Proceedings of the National Academy of Sciences,2005,102(35): 12383-12388.

[57]LAZOPOULOS K A, STAMENOVIC D. Durotaxis as an elastic stability phenomenon[J].Journal of Biomechanics,2008,41(6): 1289-1294.

[58]RENS E G, MERKS R M H. Cell shape and durotaxis explained from cell-extracellular matrix forces and focal adhesion dynamics[J].IScience,2020,23(9): 101488.

[59]ESCRIBANO J, SUNYER R, SNCHEZ M T, et al. A hybrid computational model for collective cell durotaxis[J].Biomechanics and Modeling in Mechanobiology,2018,17(4): 1037-1052.

[60]HASSAN A-R, BIEL T, KIM T. Mechanical model for durotactic cell migration[J].ACS Biomaterials Science & Engineering,2019,5(8): 3954-3963.

[61]CHAN C E, ODDE D J. Traction dynamics of filopodia on compliant substrates[J].Science,2008,322(5908): 1687-1691.

[62]BANGASSER B L, ROSENFELD S S, ODDE D J. Determinants of maximal force transmission in a motor-clutch model of cell traction in a compliant microenvironment[J].Biophysical Journal,2013,105(3): 581-592.

[63]BANGASSER B L, SHAMSAN G A, CHAN C E, et al. Shifting the optimal stiffness for cell migration[J].Nature Communications,2017, 8(1): 15313.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 649
HTML全文浏览量: 90
PDF下载量: 126
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

细胞趋硬性迁移的研究进展

doi: 10.21656/1000-0887.420265

作者简介:
杨月华（1992—），男，特任副研究员(E-mail: hz293033@ustc.edu.cn);姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

通讯作者:
姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

计量

Research Advances in Cell Durotaxis

计量

目录

留言板

细胞趋硬性迁移的研究进展

doi: 10.21656/1000-0887.420265

作者简介: 杨月华（1992—），男，特任副研究员(E-mail: hz293033@ustc.edu.cn);姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

通讯作者: 姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

计量

出版历程

Research Advances in Cell Durotaxis

计量

出版历程

目录

作者简介:
杨月华（1992—），男，特任副研究员(E-mail: hz293033@ustc.edu.cn);姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).

通讯作者:
姜洪源（1979—），男，教授，博士生导师(通讯作者. E-mail: jianghy@ustc.edu.cn).